Heterogeneity of radiation- and TNF-alpha-induced programmed cell death in carcinoma, sarcoma and lymphoma cell lines.
نویسندگان
چکیده
AIM To characterize the interaction of tumour necrosis factor alpha (TNF-alpha) and ionising radiation in six sarcoma, lymphoma and carcinoma cell lines. MATERIALS AND METHODS Cells were characterized regarding annexin V/propiduim iodine affinity and caspase-3 status after application of TNF-alpha, radiation, or combined treatment. RESULTS Three cell lines showed similar results with additive effects of TNF-alpha and radiation in both assays. The other three cell lines significantly differed regarding the detection of apoptotic cells between treatment conditions (radiation and/or TNF application) and between the two apoptosis assays. CONCLUSION The interaction between TNF-alpha and radiation differs between tumour entities and cannot be described by using only one parameter.
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ورودعنوان ژورنال:
- Anticancer research
دوره 30 7 شماره
صفحات -
تاریخ انتشار 2010